The major issues influencing human health in developing countries is the nutrient deficiency in different field crops. Several problems with the low efficiency of using the traditional chemical fertilizers by plants, despite there are more development in agricultural cultivation. Therefore, biofortification with nano-fertilizers and nano-biostimulants have been proposed as potential ways to enrich diets with nutrients. Organic fertilizers confront the issue of extending the duration of nutrient release and decreasing mineral content; however, inorganic fertilizers suffer from volatilization and loss of leaching. Nanotechnology’s application in agriculture has enormous promise to address these issues. The use of nanoparticles in biofortification of wheat is gaining traction due to their capacity to provide the optimal amount of fertilizer at the optimal time, hence reducing environmental concerns, facilitating nutrient uptake and absorption, and increasing grain production and profitability. Wheat is a major field crop that can solve with applying nano-biofortification the problem of malnutrition and nutrient deficiencies in humans. Although numerous research experiments on nano-bio-fortification of wheat plants have been conducted, this review article summarizes the benefits and results of nano-bio-fortification for grain maximization through a comprehensive review of nanomaterials and fertilizers and their effective role in bio-fortification and grain yield maximization. Moreover, current trends in the usage of many nanotechnology-based biofortification methods for wheat production were highlighted. Several methods for wheat improvement, including macro and micronutrient supply, are reviewed in more details. Understanding several features of the safe use of nanoparticles and their future prospects may assist in their effective use in terms of economics and nutritional needs after the usage of nano-fertilizers and nano-biostimulants in biofortification and wheat grain yield maximization.