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Objective: To see the efficacy of rapid color test for the sensitivity of ceftriaxone against clinical isolates of Salmonella Typhi. Methods: This descriptive cross sectional study was carried out at the department of Microbiology, Pakistan Navy Ship (PNS) Shifa Hospital, Karachi from Nov 2018 through April 2019.We tested 97 clinical isolates of Salmonella Typhi by this rapid color test. All the isolates were also test by conventional disc diffusion method and MICs by Vitek-2 system. Results: Out of these isolates, 83 were ceftriaxone resistant while 14 isolates were ceftriaxone sensitive. Sensitivity and specificity of rapid color test were 100% when compared with results of disc diffusion, E-test and Vitek 2 system. All the results were readable within 2 hours on color test. Conclusion: This color test is rapid, accurate and inexpensive and can be used as a screening test for ceftriaxone resistance in typhoid endemic areas. Keywords: Ceftriaxone; drug resistance; meropenem Continuous...

Objective: To evaluate the antibiotic resistance pattern of Pseudomonas aeruginosa and its prevalence from samples received from Intensive Care Units (adult and neonatal) at PNS Shifa Hospital Karachi. Study Design and Setting: This cross sectional study was carried out at PNS SHIFA hospital. Methodology: Samples including blood, pus, wound swab, sputum and endobronchial washing were received for culture and sensitivity. Isolates were cultured on blood and MacConkey agar. Antibiotic susceptibility testing was performed by Kirby-Bauer and broth microdilution and then analyzed on SPSS version 23.Results were confirmed by VITEK 2. Results: Out of 674 positive clinical specimens 97(14.39%) were positive for Pseudomonas aeruginosa growth. The most susceptible antibiotic against Pseudomonas aeruginosa was Polymixin b (94.854%).The least effective antimicrobial was aztreonam (40.21%sensitive). Conclusion: The prevalence of Pseudomonas aeruginosa from the samples of Intensive Care Units was found to be 14.39%. The most susceptible antibiotic against pseudomonas was Polymixin B. The least effective antimicrobial was aztreonam