Searching for "intelligence": Azurin-PNIPAM conjugates were prepared by site-directed mutagenesis followed by protein reconstitution by using imidazole-conjugated poly(N-isopropylacrylamides). The polymer-bound imidazole acts as a ligand in the active site of the blue copper protein azurin. The bioconjugates showed thermosensitive behavior in electron-transfer processes with reduced cytochrome c.
Azurin‐PNIPAM‐Konjugate wurden durch zielgerichtete Mutagenese und Proteinrekonstitution mit Poly(N‐isopropylacrylamiden), die Imidazol‐Endgruppen tragen, hergestellt. Das polymergebundene Imidazol bindet an das aktive Zentrum des Kupferproteins Azurin. Die Biokonjugate zeigen temperaturabhängiges Verhalten in Elektronentransferprozessen mit reduziertem Cyctochrom c.
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