Abstract:Since the level of job motivation and associated factors among health care professionals in public health institutions in Gedeo Zone is not yet studied the objective of this study is to assess Level of job motivation and associated factors among health care professionals in public health institutions of the Zone. A cross-sectional study design was conducted in 36 health center of Gedeo zone, during February 20 to May 10, 2015. The data were analyzed using SPSS. Factor score was computed for the items identified to represent the level of job motivation. Using this regression factor score, multivariate linear regression analysis was performed. More than three quarter 77.4% (226) of the respondents were discourage from working hard for different reasons. only 57 (19.5%) of Health care professionals working in public health centers were highly motivated, only 6.2% (18) of them are rewarded for their hard working. Sex, communication, resource availability, inadequate salary, feedback, training, working environment, workload and recognition were negative determinates of job motivation. The motivation of Health professional in Gedo zone is low. Since half of the professionals were demotivated, healthcare organizations should maximize its effort to practice incentive schemes.