The survival and proliferation of free and alginate immobilized, Azospirillum brasilense in a bulk soil collected from Derna region at different moisture content levels (10%, 40% and 60 %). A reduction of cell number in the experimental units (sterile or non-sterile soil) for all the tested moisture levels was observed after 15 days after the inoculation. Nevertheless, different parameters among the level of moisture content could be observed in the reduction of the population .soil-sterilization did not affect Azospirillum survival and proliferation, and results presented in results show that Azospirillum brasilense total content was not affected by the sterility of the soil. In general, Azospirillum brasilense strain isolated from Derna soil survived well and for long period (75day) in bluk soil (without plant roots). In this study the presence of moisture in the soil as well as the soil- type of Derna which is rich in organic matter maintained Azospirillum brasilense population of a level high enough to be detected 75 days after inoculation.

The Screening of three isolates of Trichoderma (T.  ghanense, T. harzianum and T. koningii) for Carboxymethyl cellulase (CMC-ase) production. Whereas, T.  koningii was the most potent isolates for enzyme activity. Trichoderma, koningii produced CMCase at all the tested levels of   benomyl (C14H18N4O3) and enzyme production with entrapped fungal species was not affected by the addition of benomyl at concentrations 5, 10, 15 and 20 μg a.i / ml. Enzyme production was improved by the alginate encapsulation as compared with conidia suspension. Alginate entrapped conidia of Trichoderma koningii was successfully used in 3-repition for CMC-ase production.

Azospirillum was abundant in the rhizosphere of halophytes collected from El- Thaliga salt marsh near Benghazi, Libya. More than twenty five isolated of Azospirillum were isolated from rhizosphere by enrichement method using DN semisolid medium with or without salt. After several transfer for purification, 13 isolates were screened to select the most efficient strains in a microcosms experiment with wheat. According to the cultural, morphological and biochemical experiments Azospirillum isolated were belonging to A. brasilense and isolated was belonging to A. lipoferum, Azospirillum isolates were screened for growth at different NaCl concentration in liquid DN medium.