Haritakyadi eye drops are prepared by hydro-distillation (Arka kalpana) of ingredients like Terminalia chebula Retz. (Haritaki), Emblica officinalis Linn. (Amalaki), Berberis aristata Dc. (Daruharidra) and Glycyrrhiza glabra Linn. (Yastimadhu). The research was designed to explore the volatile constituents by Gas Chromatography-Mass Spectrometry (GC-MS). Secondary metabolite screening using GC-MS revealed the presence of fifteen constituents. Out of fifteen constituents 8 could not be identified and remaining 7 compound identified as 1

An open label clinical trial was conducted to evaluate the efficacy of Haritakyadieye drops on Kukkunakaw.s.r to Ophthalmia Neonatorum in newborns at Sri Dharmasthala Manjunatheshwara College of Ayurveda and Hospital, Kuthpady, Udupi. This trial was conducted on 30 newborns. The drops were instilled 8th hourly in a 3 divided doses per day for 3 days. The response was assessed by using various subjective and objective parameters and analysed statistically using Wilcoxon Signed Rank Test. Statistically significant results were obtained in subjective parameter and conjunctival swab culture. Study showed Haritakyadi eye drops is effective in relieving the clinical symptoms as well as controlling the growth of gram positive and negative organisms.