Aim:The main objective of this project is to enhance the Bandwidth of microstrip patch antenna by using an E-shaped slot on the patch and varying substrate thicknesses in comparison with the Rectangular patch antenna. Materials and Methods: The microstrip patch antenna with an E-shaped slot and with varying substrate thicknesses of four groups [1.2mm, 1.6mm, 1.8mm, 2.4mm] is used with a sample size of 40 in comparison with rectangular patch antenna of another group with sample size 10. Results: By using Altair Feko simulation software, the proposed antenna parameters such as Bandwidth and Gain are obtained. The results are Gain=9dB, Bandwidth=225MHz for 1.2mm, Bandwidth=250.1MHz at 1.6mm, Bandwidth=252MHz at 2.4mm substrate thicknesses and bandwidth of conventional Rectangular patch antenna of 1.6mm substrate thickness=243MHz. Attained Significance accuracy ratio p(<0.05). Conclusion: The bandwidth appears to be enhanced (12%) by varying the substrate thicknesses [1.2mm to 2.4mm] from 225MHz to 252MHz.