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A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.

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ABSTRACTthe Insulin-Like Growth Factor 1 plays a key role in foetal development and post natal growth. the objectives of this study were to characterise the complete coding sequence of caprine IGF1 gene in two indigenous goat breeds of India: Malabari and attappady Black, to detect polymorphisms of IGF1 gene, to investigate their effects on body size traits and to ascertain the relative expression of IGF1 mrNa in muscle tissues of goats belonging to low and high body weight groups. All the four exons of caprine IGF1 gene were amplified and characterized by PCr-SSCP in 298 goats, revealing two genotypes (CC and Ct) at exon 2. Sequencing of the PCr products from each genotype revealed a novel SNP, g.80C>t (GenBank accession No. kM974180), which caused a non-synonymous mutation (thr48Met),causing differences in IGF1 protein structure. association analysis of the loci indicated Ct genotypes have higher body length (P<0.01), chest circumference (P<0.01) and body length index (P<0.05) than CC genotypes. two novel PCr-rFLPs were designed for the rapid detection of the genotypes. the quantitative real time PCr demonstrated a difference in the expression of IGF1 mrNa in muscle tissues of the low and high body weight groups, but it was not significant (P>0.05). The results of the *Corresponding author: Dr. Naicy thomas, M.V.Sc., PhD, assis. Prof., Department of animal Breeding, Genetics and Biostatistics, College of Veterinary and animal Sciences, Mannuthy, thrissur-680651, kerala, India, Phone: +91 94 4611 9307; e-mail: naicy@kvasu.ac.in 458Vet. arhiv 87 (4), [457][458][459][460][461][462][463][464][465][466][467][468][469][470][471][472] 2017 t. Naicy et al.: association of IGF1 gene polymorphism with phenotypic variants in goats present study suggest that the alleles of the IGF1 gene could be considered as strong targets for improvement of growth traits in goats.

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