Background: Breast cancer is common cause of cancer death among women with a complex and heterogeneous picture in histological, molecular and clinical features. The aim of this study was to identify hub genes and related pathways in breast cancer.Methods: We selected screening methods for differentially expressed mRNAs and miRNAs using expression profile data of Breast cancer from The Cancer Genome Atlas (TCGA). with using some databases for annotation, the functional and pathway enrichment for differential expression genes (DEGs) was performed. We selected genes and miRNAs with differential expression pattern. Then we determined target genes for differential expression miRNAs (DEMIs) and intersection between them was selected as differentially expressed miRNA-target genes for breast cancer. In the next step we constructed miRNA-mRNA regulatory network and protein–protein interaction (PPI) network for more information.Results: Top 10 DEMIs were identified from miRNA profile. Then, we selected 354 gene as target gene for 10 DEMIs. The miRNA-mRNA and PPI network constructed and 10 hub genes and 5 miRNAs were identified that some of them are new for breast cancer. Also, miRNA-target genes with differential expressions in this study were all mainly involved in signaling pathways and developmental process.Conclusion: This study identified some candidate biomarker for breast cancer that they have a potential role in pathways related to breast. These findings can be used for research, early diagnosis and therapeutic goals.