Background: Apoptosis inhibition depends on the balance between expression of some regulatory genes including Bax and c-FLIP, despite breast cancer has long been associated with disturbances in apoptosis regulation. The objective of present study was to determine the expression levels of Bax and c-FLIP mRNAs in 1 μg sample of total RNA obtained from MCF7 and MDA-MB-231 human breast cancer cells treated with Prunus armeniaca extract. Methods: Prunus armeniaca effect was measured by MTT assay with different drug concentrations (0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5 mg/mL) at different incubation times (24, 48 and 72 h). Expression of Bax and c-FLIP mRNA levels was determined by qRT-PCR technique. Results: The results of this study indicated that Prunus armeniaca significantly inhibited proliferation of cells in concentration-dependent manner in all the time of incubation (P < 0.05). Our findings also showed that the expression level of Bax and c-FLIP genes were increased in untreat group compared with control group in both of cancer cells at all the time (P < 0.001). Prunus armeniaca reduced the expression level of Bax and c-FLIP genes cancer cells as a timedependence manner with respect to the untreat group significantly (P < 0.001). Conclusion: The expression level of Bax and c-FLIP genes were significantly decreased under treatment of Prunus armeniaca extract and it may be helpful in the prevention and cure of breast cancer in usage of herbal remedies.

Background and Objective: The SOX2OT lcnRNA has been recognized as positive regulator in transcription regulation of SOX2 gene. Recent studies have approved the dysregulation of SOX2OT lncRNA expression patterns in some common caner types including esophageal, lung, and breast cancer. The objective of present study was to investigate correlation between overexpression of SOX2OT lcnRNA and susceptibility to breast cancer. Method: SOX2OT lncRNA expression profiling in 15 breast cancer and normal tumour-adjacent breast tissue samples was performed by using qRT-PCR. To evaluating the diagnostic potential of the SOX2OT lncRNA we performed ROC curve analyses. Results: The expression of SOX2OT lncRNA in patients suffering from breast cancer revealed a significant overexpression in comparison with the healthy group (P<0.001). Significantly, the elevated circulating SOX2OT lncRNA was found specific to breast cancer and could differentiate breast cancer from controls with 100% of both sensitivity of and specificity. Based on the Kaplan-Meier analysis, there were no significant correlation between SOX2OT lcnRNA expression and overall survival. Conclusion: The results confirmed the association between breast cancer and higher SOX2OT lncRNA expression. According to the ROC curve results, SOX2OT lcnRNA could be a new measurable indicator of the breast cancer and a potential therapeutic target for breast cancer patients.

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