Colostrum is an arsenal of proteins and peptides required at the earliest stage of a newborn development. Proteins involved in all biological processes of an infant development have been found in its composition. It is expected as cost-effective source of biologically active proteins and peptides. In this work, we separated water-alcohol-soluble low abundant proteins from bovine colostrum in preparative amounts that were further utilized in combination with amaranth oil for topical cream composition. The ratio of the obtained proteins’ fraction made a thousandth of the colostrum dry mass. The partial sequences of 37 identified proteins were established by mass-spectrometer and using BLAST search in NCBI database. In our previous work, we established the chemical composition of amaranth seed oil with ~6 % squalene by mass. The physical mixtures of these natural resources were fabricated into cream using hyaluronic acid as moisturizing agent and their analgesic activities were established. The optimal ratio of proteins and oil was determined in terms of their effects as analgesic means by experiments carried out on mice. Several proteins could possibly be responsible for the revealed biological efficacy. Among them, G-protein coupled receptor and synaptotagmin were previously linked with analgesic activity. Establishing an optimum ratio of ingredients proved also the contribution of higher quantity of amaranth oil, a rich source of squalene and unsaturated fatty acids.