The aim of this study was to evaluate the co-occurrence of some frequent fecal indicator bacteria and cyanobacteria species at San Roque reservoir (SRr), highlighting the relationship between their variability and environmental factors. This study was carried out at SRr (31 22 0 S, 64 27 0 O) located west of the city of Cordoba, Argentina. Physico-chemical measurements and sampling for microbiological and nutrient determinations were done at three recreational sites, during seasons of major touristic activity (springsummer), with the aim of identifying temporal patterns of microorganism variability. In addition, we carried out laboratory experiments with local isolated bacteria (Escherichia coli and Enterococcus spp.) and axenic Microcystis aeruginosa cultures to assess an association among these microorganisms and evaluate whether it was positive or negative. Our results showed an inverse relationship between cyanobacteria and thermotolerant coliform bacteria (TtC) at SRr, which was only observed when high cyanobacteria concentration occurred (!3.4 Â 10 4 to 1.2 Â 10 6 cell.mL À1). Experimental results also showed this type of relationship: E. coli and Enterococcus spp. decreased their growth in the presence of M. aeruginosa. In addition, it was observed that the variability of main phytoplanktonic species and TtC at SRr was mainly explained by temporal patterns, that is, the seasonal changes and the hydrological year. In summary, our data indicated that cyanobacteria high abundance during bloom periods could be an inhibiting factor for bacteria growth at SRr. Moreover, we demonstrated that nutrients such us P and N are not the main factors determining the variability of studied microorganisms in this eutrophic reservoir, but other factors, such as climatic conditions (temperature and precipitations), have a major influence. This study would provide a more comprehensive view on the dynamics of microorganism populations with sanitary relevance in eutrophic reservoirs.
The objective of the present work was to determine the value of the longitudinal dispersion coefficient (DL) of the Chicamtoltina stream (Alta Gracia) by means of two different techniques, in order to compare the values obtained. The first technique consisted of applying a developed formula that includes a detailed description of hydrodynamic parameters obtained by gauging with a hydroacoustic instrument, while the second technique consisted of injecting a conservative tracer, using the same approach as the non-ideal chemical reactor theory of flow with dispersion. This work was carried out at low flow conditions (dry period) and at high flow conditions (wet period). It was found that, either for high flow or low flow, the values of the dispersion coefficient obtained by both techniques have good agreement, fitting better in the dry period than in the wet period. Due to the fact that frequent gauging campaigns are carried out in this stream, it is concluded that with similar flow characteristics and morphology of the section, the gauging data can be used to determine the DL coefficient, in order to incorporate reliable data that can be applied to pollutant transport models.
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