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A new liquid chromatographic method was proposed for the estimation of the amount of water which exhibits selectivity to solutes in water-swollen hydrogels based on the model regarding the retention of ions on the column packed with the hydrogels. The estimated amount of the water in polyacrylamide, dextran, and poly(vinyl alcohol) gels corresponds to the sum of the amount of freezable bound water and that of nonfreezing water determined by means of differential scanning calorimetry. This means that the freezable bound water takes an important role in the separation process using water-swollen hydrogels contrary to the conventional view of the freezable water.

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