IB and ELISA of the NC16a domain of BP180 were shown to be sensitive and diagnostic methods in PG. Patients with PG rarely reacted with BP230, suggesting a different pathogenesis between PG and bullous pemphigoid. Multigravidae developed PG skin lesions significantly earlier in pregnancy than primigravidae.
Stem segments with axillary buds of Glycyrrhiza glabra L. were cultured on modified MS medium supplemented with a-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). Formation of multiple shoots was obtained on the medium provided with 1.00 mg/l BAP after 40 days of culture.Each shoot was cut and transplanted onto the medium supplemented with NAA and BAP. After 80 days of culture, the rooting of shoots was well established in the media containing 0.01-0.50 mg/l NAA and these shoots subsequently developed into whole plants.
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