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Our search for new metabolites biosynthetically related to fusicoccin with potent plant growth-stimulating actiyity from the culture Mtrate of fusicoccum amJtgtinli F6 resulted in the isolation of a new deriyatiye of fusicoccin J, as well as fusicoccin J and 16-O-demethyl 3-epifusicoccifi J. Its structure was analyzed by 600 MHz NMR spectrometry and identified as 16-O-demethyl fusicoccin J. The biosynthetic relationships among 16-O-demethyl fusicoccin J and its 3-epimer, and f-sicoccin J are discussed in connection with the co-occurrence of both of the 3-epimers of 16-O-demethyl fusicoccin J. 16-O-Demethyl fusicoccin J alld its 3epimer showed oilly low germination-stimulatillg activity toward lettuce seeds in the presence of abscisic acid (20 "M), the latter being slightly mere actiye than the former.