In the present study, the aflatoxin B1 contamination at various stages of oil refining and in refined oil were carried out. This was subsequently compared with commercial vegetable oil samples. Among the 23 different sunflower oil samples were tested, 10 of them showed posi-tive results to AFB1 and the remaining 13 showed negative results to AFB1. All the refined oil samples were free from AFB1 contamination
Accessibility and availability of fresh clean water is a key to sustainable development and essential element in health, food production and poverty reduction. In the present study, we have collected water sachet containing CM/L number and they were analysed for physical and bacteriological nature. The organisms isolated in this study were Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas vesicularis and Pseudomonas aeruginosae. The harmful effects of these isolates were evidenced by antibiotic resistance, heavy metal tolerance and antibacterial activity. They were resistant to the antibiotics like amoxiclav, methicillin, chloramphenicol and streptomycin. They showed tolerance to the heavy metals at 5 mM conc. except for lead. For antibacterial activity, they were tested against human pathogens Klebsiella pnemoniae, Proteus mirabilis, Micrococcus leuteus and Salmonella paratyphium. But at the same time these organisms could be exploited for the industrial production of amylase, protease and cellulase.
Aflatoxins are the potent toxic, mutagenic, heterogenic and carcinogenic metabolites produced by species of A. flavus and A. parasiticus. In the present study, an attempt has been made to prevent aflatoxin production using an anticancerous drug taxol. Taxol (Paclitaxel) is a well known drug for its anticancerous property mainly to treat breast and ovarian cancers. It was obtained from Taxus brevifolia and it was also obtained from the endophytic fungi present in Taxus brefivolia [1]. Therefore, this drug is specifically selected to screen its activity on the control of A. flavus and AFB1 production at various concentrations. Among the 6 concentrations used, 3 μg of taxol was found to be suitable to control the growth and AFB1 production. The content of AFB1 found at this concentration was 6 ppm by TLC and 6.3 ppm by HPTLC. The complete elimination of AFB1 might require higher concentrations of taxol
The inhibition efficiency (IE) of caffeine in controlling corrosion of carbon steel in well water in the absence and presence of Zn2+has been evaluated by mass loss method. The formulation, consisting of 200 ppm of caffeine and 50 ppm Zn2+, offers 82% inhibition efficiency to carbon steel immersed in well water. Addition of malic acid increases inhibition efficiency of the caffeine-Zn2+system. The inhibition efficiency of caffeine-Zn2+and caffeine-Zn2+-malic acid system decreases with the increase in immersion period and increases with the increase in pH from 3 to 11. AC impedance spectra, SEM micrographs, and AFM studies reveal the formation of protective film on the metal surface. The film is found to be UV fluorescent.
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