Bioleaching of metal sulfides is an interfacial process where adhesion and subsequent biofilm formation are considered to be crucial for this process. In this study, adhesion and biofilm formation by several acidophiles (Acidithiobacillus, Leptospirillum and Sulfobacillus) isolated from different biotopes with sulfide ores in Armenia were studied. Results showed that: (1) these bacteria adhere to pyrite surfaces to various extents. A correlation between pyrite biooxidation and adhesion of S. thermosulfidooxidans 6, L. ferriphilum CC, L. ferrooxidans ZC on pyrite surfaces is shown. It is supposed that bioleaching of pyrite by S. thermosulfidooxidans 6, L. ferriphilum CC, L. ferrooxidans ZC occurs by means of indirect leaching: by ferric iron of bacterial origin; (2) cells of At. ferrooxidans 61, L. ferrooxidans ZC and St. thermosulfidooxidans 6 form a monolayer biofilm on pyrite surfaces. The coverage of pyrite surfaces varies among these species. The order of the biofilm coverage is: L. ferrooxidans ZC ≥ At. ferrooxidans 61 > St. thermosulfidooxidans 6; (3) the extracellular polymeric substances (EPS) analysis indicates that the tested strains produce EPS, if grown either on soluble ferrous iron or solid pyrite. EPS are mainly composed of proteins and carbohydrates. Cells excrete higher amounts of capsular EPS than of colloidal EPS. In addition, cells grown on pyrite produce more EPS than ones grown on ferrous iron.
Possibilities for the recovery of non-ferrous and precious metals from Kapan polymetallic mine tailings (Armenia) were studied. The aim of this paper was to study the possibilities of bioleaching of samples of concentrated tailings by the natural microbial consortium of drainage water. The extent of extraction of metals from the samples of concentrated tailings by natural microbial consortium reached 41-55% and 53-73% for copper and zinc, respectively. Metal leaching efficiencies of pure culture Leptospirillum ferrooxidans Teg were higher, namely 47-93% and 73-81% for copper and zinc, respectively. The content of gold in solid phase of tailings increased about 7-16% and 2-9% after bio-oxidation process by L. ferrooxidans Teg and natural microbial consortium, respectively. It was shown that bioleaching of the samples of tailings could be performed using the natural consortium of drainage water. However, to increase the intensity of the recovery of valuable metals, natural consortium of drainage water combined with iron-oxidizing L. ferrooxidans Teg has been proposed.
Agrotourism is developing very fast worldwide, and Armenia is not left out of those rates of development. There are serious preconditions for the development of agro-tourism in Armenia. This study aims to reveal the current state of agro-tourism, outlining the development preconditions in Armenia, particularly the role of PR in the development of this field. To collect the necessary information, we used the methods of expert and in-depth interviews, document analysis and viewing.
The studied data refer to gastro-houses, house-studios, adventure tourism and other interesting directions of agro-tourism, which were spread in the Republic of Armenia until the first half of 2020 and were in different regions, in particular: Gegharkunik, Vayots Dzor, Shirak, Lori, Kotayk, Tavush, Aragatsotn, Ararat.
The results of the research allow us to conclude that:
• Agro-tourism has prospects for development in Armenia;
• Agro-tourism can contribute to the development of different villages and communities in the Republic of Armenia;
• Planning PR campaigns is a necessary precondition for development;
• The lack of a common tourism platform in the Republic of Armenia hinders the development.
• The expansion of packages offering rural tourism by Armenian tour operators will contribute to development.
• It is possible to create a network of guest houses, which will include only the ones that are engaged in agro-tourism.
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