Introduction: Oxidative stress is a condition in which the reactive oxygen species production exceeds the antioxidant system capacity to neutralize these peroxidases. In these situations, proteins, lipids, and nucleic acids are damaged. In this regard, the cherry can be noted as a food antioxidant which leads an increasing antioxidant capacity and reducing inflammation and damage muscle. Therefore, The purpose of this study was to determine the effect of cherry juice supplementation on total antioxidant capacity (TAC), creatine kinase (CK), hydrogen peroxide (H 2 O 2 ) in non-athlete men after an exhaustive aerobic exercise. Method: In this quasi-experimental research, ten untrained (UT) men were randomly selected. Then, they were divided into two equal groups: supplement group (cherry juice) and placebo group (commercial Cherry juice diluted with natural water). After eight days of supplementation period, all subjects were participated in aerobic exercise protocol (Bruce test run to the point of exhaustion) on the treadmill. Primary blood samples in the baseline were taken. The second was immediately after the Bruce test, third and fourth were six and twenty-four hours later were taken (5 ml). For analysis of the results. Analysis of variance with repeated measures was used at the significant level. Result: A significant effect of short-term cherry juice supplementation on TAC, H 2 O 2, and CK was observed (p≤0.05). Conclusions: In general, it can be concluded that probably eight days of cherry juice supplementation probably cannot prevent the adverse effects of oxidative stress caused by acute aerobic exercise.