Minichromosomal Maintenance protein 7 (MCM7), a member of the MCM family of proteins critical to the DNA replication complex, has recently been shown to improve the sensitivity and specificity of Epithelial Ovarian Cancer (EOC) diagnosis but its function in Carcinogenesis is not clear. We evaluated MCM7 gene expression, RMI and CA125 serum level as diagnostic tools of primary ovarian cancer in Egyptian women. The MCM7 gene expression was evaluated by SYBR green Quantitative Real Time PCR (Q RT-PCR) in ovarian tumor tissues of 50 Egyptian women. 25 malignant and 25 benign tumor tissues were studied. Serum Human cancer antigen 125 (CA 125) was measured in the serum of all participants of the study using immune sorbent assay (ELISA). The MCM7 showed significant difference among ovarian malignant tumors patients compared to the control subjects (p< 0.01). Absolute sensitivity of MCM7 & 96% specificity were found with the best cutoff value at 1.96 .There was a significance correlation between MCM7 with RMI and with CA125 in all patients of the study (p<0.01 for both). The mRNA expression of MCM7 was positively correlated with the progression of the stage & grade of the tumor (p <0.01) for both.