HIS study aimed to isolate and identify Salmonella spp. From human and chickens by using culture method, serotyping, antimicrobial sensitivity test and detection of virulence genes (invA, avrA, bcfC, stn). A total of 245 samples and swabs including (36 cloacal swabs, 17 intestinal contents, 60 chicken product[ luncheon, nuggets , kofta, pane, 15 of each], 45 chicken meat [ breast, thigh, wing, 15 of each], 20 hand swabs , 27 stool (diarrheic and nondiarrheic ) and 40 serum) from humans for Widal test. The results revealed that 31 samples and swabs were positive to Salmonella, 6 isolates were isolated from broilers cloacal swabs, 7 isolates were isolated from chicken meat (4 in thigh samples and 3 in breast samples and swabs), 2 isolates were isolated from chicken products (one isolate in kofta sample and one isolates in pane swabs), 3 isolates were isolated from intestinal swabs, 4 isolates were isolated from hand swabs from workers in farms and poultry shops and 4 isolates were isolated from stool of diarrheic and non-diarrheic persons while 5 isolates were isolated from serum. Serotyping revealed 9 strains of S.