Background: Can1 is a yeast plasma membrane permease catalyzing specific uptake of arginine. Results: Two residues in the binding pocket of Can1 affect its selectivity. Conclusion: Subtle amino acid changes can convert Can1 to a lysine-specific permease. Significance: Understanding, at the molecular level, the translocation mechanism(s) of yeast amino acid transporters has bearings on our knowledge of other transporters featuring the same fold.
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