No abstract

Classic dye injection methods yielded amazingly detailed images of normal and pathological development of the cardiovascular system. However, because these methods rely on the beating heart of diffuse the dyes, the vessels visualized have been limited to the arterial tree, and our knowledge of vein development is lagging. In order to solve this problem, we injected pigmented methylsalicylate resins in mouse embryos after they were fixed and made transparent.

Injection of colored methyl salicilate into the atria of fixed mouse embryos reveal the pattern of developing veins. See Délot et al., Developmental Dynamics 236:2586–2593. © 2007 Wiley‐Liss, Inc.