This study aimed 1) to develop an e-learning based on the microlearning approach and 2) to examine quality of the developed e-learning. To examine the quality, three research instruments including pretest and posttest, a perception survey questionnaire and a quality evaluation form were used. The sample group consisted of 21 Thai undergraduate students in Faculty of Education at Chulalongkorn University. Descriptive statistics which were mean and standard deviation (SD) and one sample t-test were employed for the data analysis. The findings from one sample t-test, at a significance level of .05, illustrated the students’ noteworthy improvement of the pronunciation skills after receiving the treatment. Furthermore, the results from the perception survey questionnaire revealed their positive perception towards the e-learning. The experts' evaluation score also reflected its high-quality level. The study could be finally concluded that developing and using the microlearning-based e-learning is effective for the English phonetics and pronunciation class.