No abstract

32Understanding spatial gene expression and regulation is key to uncovering developmental and 33 physiological processes, during homeostasis and disease. Numerous techniques exist to gain gene 34 expression and regulation information, but very few utilise intuitive true-to-life three-dimensional 35 representations to analyze and visualize results. Here we combined spatial transcriptomics with 3D 36 modelling to represent and interrogate, transcriptome-wide, three-dimensional gene expression and 37 location in the mouse adult heart. Our study has unveiled specific subsets of genes that display 38 complex spatial expression in organ sub-compartments. Also, we created a web-based user interface 39 for spatial transcriptome analysis and visualization. The application may be accessed from