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Secondary metabolite contents of W. somnifera varied remarkably between seasons and genotypes under ex vitro condition. In vitro studies provide an optimum culture condition for steady and quality production of bioactive chemicals throughout the year without involvement of environmental stresses. Mass production of micro-shoots and plantlets by exploring the organogenic totipotency of shoot tip explants (ex vitro and in vitro grown) considering two elite genotypes (Poshita and Jawahar 22) of W. somnifera, and assessment of their capability in production and accumulation of bioactive metabolites (total alkaloid and withanolides amount were quantified; withanolide A and withaferin A contents were estimated by High Performance Liquid Chromatography-HPLC).

Datura stramonium is a well-known traditional herb with therapeutic properties. It is despicablygrown wildly and has both noxious as well as medicinal properties. This herb has proven tohave very great pharmacological prospects with great utility in old medical practices as well asin the current medical field. It has been scientifically confirmed that D. stramonium containsalkaloids, also known as belladonna alkaloids. In the near future, this herb will be used to treata variety of other human diseases. This herb has contributed to many pharmacological activitiesin the scientific discipline worldwide and the Indian medical system, including anti-asthmaticaction, anti-inflammatory activities, anti-cholinergic activities, etc. In-vitro micropropagationis being practiced to grow a large number of datura plants to produce high-quality alkaloidsnaturally for the pharmaceutical industry which has not been practically realistic in the past.

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