Aim: This study aimed to investigate the pathological effects of the infectious bronchitis virus (IBV) on chicken trachea and kidney tissues and also desired to diagnose the virus genome using a molecular tool. Materials and Methods: Twenty trachea and kidney samples collected from one broiler farm contain 10,000 chickens at Tikrit city. The chickens showed signs of gasping and mortality (20%) at early ages (20 days old), the presence of IBV investigated using conventional reverse transcriptase-polymerase chain reaction technique with routine histopathological study to tracheal and renal tissue. Results: Postmortem lesion showed severe respiratory inflammation with abscesses at tracheal bifurcation lead to airway blog. Molecular results showed two genotypes of IBV, one of them not included in primer designer research. The histological study showed different stages of inflammation, degeneration, and necrosis to the renal and tracheal tissues. Conclusion: The respiratory and renal pathological effect of the virus responsible for the symptoms appeared on the affected chicks that caused mortality, with a high probability of presence of a new viral genotype added to the untranslated region.

The study aimed to determine the effect of S. cerevisiae and L. acidophilus as probiotic against S. typhimurium isolated from poultry, for this purpose (50) fecal samples were collected from poultry to isolated S. typhimurium, while fermented milk used for isolation of Lactobacillus acidophilus. Results of current study showed that S. typhimurium isolated in rate of 6%. In-Vivo adhesion Index test showed high ability of L. acidophilus to adhesion on rat intestine endothelium in compare with Saccharomyces cerevisiae .The inhibitory zone occurred by S.cerevisiae filtrate, L. acidophilus filtrate, (S .cerevisiae and L. acidophilus mixture) filtrate were (10, 16, 19 mm) respectively. The results of experimental study showed that high activity of (S.cerevisiae and L. acidophilus mixture) filtrate in protect experimental animals. Main pathological changes occurred by S.typhimurium were infiltration of inflammatory cells.

T HIS study carried out in Salah aldeen province in period from January to September 2019. The aims of this study were to investigate incidence of Salmonella typhimurium and Salmonella dublin in local Iraqi cows by using of PCR test.The results of current study showed that the rate of isolated Salmonella species was 13.3% by culture methods, highest of them from aborted cows in rate of 22.2%.PCR test detected Salmonella typhimurium and Salmonella dublin in rate of 55% and 25% respectively, while other Salmonella species has been detected in rate of 20% from total Salmonella isolates.We can concluded from this result that highly incidence of Salmonella in cow and Salmonella typhimurium is most isolated type.