The present study’s objective was to evaluate the impact of Bunium incrassatum roots’ powder (Talghouda) as a dietary supplement on the evolution of biological, biochemical, and histological parameters in female Wistar rats. The animals were divided into two groups and given an orally standard diet supplemented with 15% of Bunium incrassatum roots powder, daily for 15 days. The obtained results showed that the roots of Bunium incrassatum induced an increase in the body and organs weight of Diet rats compared to the control, as well as effects on the biochemical parameters, characterized mainly by a significant increase in the glycaemia (+44.44%), triglyceride (+56.81%), cholesterol (+11.90%), LDL (+4.08%) and HDL (+11.53%)and a decrease in creatinine (-3.65%), TGO (-6.92%) and TGP (-58.73%). In the histological study of organs, the thyroid of diet rats revealed large thyroid follicles, the colloid of the follicles was more or less developed compared to the control rats. Through the obtained results, it can be concluded that a standard diet supplemented with 15% of Bunium incrassatum roots powder may have a positive effect on biological, biochemical, and histological parameters

Adverse early life events, such as early maternal separation, may alter the normal pattern of brain development and subsequently the vulnerability to a variety of mental disorders in adulthood. Maternal separation relatively changes behavioral and biochemical responses leading to an overactivation of the hypothalamic-pituitary-adrenal (HPA) axis, and subsequently increases the ACTH level. In this study, we aimed to evaluate the role of a corticosteroid blocker, ketoconazole, in behavioral, hematological and biochemical changes in rat underwent maternal separation (MS). For this purpose, On postnatal days 1-7, rats were submitted daily to MS for 1h30min per day. At 3 months of age, rats were given a high dose of ketoconazole (200mg/kg), exploratory activity and anxiety-like behavior were evaluated using the open field test and elevated plus maze test, olfactory-like behavior was evaluated using the olfactory recogneting and biochemical and hematological profiles were also evaluated. We found that MS provoked depressive and anxiety-like behaviors, olfactory sensibility and biochemical changes (increase in level of blood glucose and ACTH) in adult rat. In addition, treatment of stressed rat by ketoconazole could modulate the above-mentioned negative effects of maternal separation"MS". In conclusion, our results demonstrated that ketoconazole decrease anxiety like behavior and regulate biochemical change induced by maternal separation stress.