BackgroundCaspase-3 and granzyme B were claimed as apoptotic manipulative enzymes.AimsThe present study was to determine the enzymes expression and activity in cancer and cancer immune therapeutic status and the possible association to cancer common pathological signs targeting the improvement of therapeutic conditions.Material and MethodsMice were immunized with cell lyaste or cell lysate + CKI in the right thigh and challenged with live cells of ehrlich ascites carcinoma (EAC) in the left thigh. The expression and activity of both enzymes in the spleenocytes derived from different subjects (normal, EAC and cell lysate or cell lysate + CKI immunized mice) after cultured with EAC viable cells were determined by colorimetric assay and western blot analysis. In addition, the subjects DNA ladder and serum metalloproteases (MMP 2 and 9) zymography were observed.ResultsThe experimental data revealed over expression of caspase3 and granzyme B in the groups of cell lysate or cell lysate + CKI immunized mice compared to control while down expression were recorded in the EAC subject. The over expression of the 2 enzymes were accompanied with increases in the activities of caspase3 and granzyme B, changes in DNA fragmentation and inhibition of metalloproteases.ConclusionIt could be suggested that, the parameter estimation within the present experimental framework could identify the efficiency of therapeutic vaccine protocols and elucidate the impact of CKI adjuvant with vaccines therapy.

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The current study is an endeavor to estimate how much the teratogenic effect of saffron (Crocus sativus L) on pregnant rats upon exposure to low doses of gamma radiation. Pregnant rats were received 50 mg saffron extract/kg body weight (every 2 days over 18 days) and exposed to 3Gy of gamma radiation delivered as 0.5Gy every 3 days over 18 days. Transcription factor HIF-1, sFLT-1, a soluble like tyrosine kinase with antiangiogenic properties, significantly increased in placental and fetus tissues of rats received saffron treatment compared to control rats. Plasma VEGF and placenta VEGF receptors (FLT-1) endure significant decreases in pregnant rats treated with saffron and significant alteration in the NO concentrations in both of placenta and fetus tissues. Deranged antioxidant GSH concentration and GSHPx activities, and pro-oxidant MDA, were markedly observed. Besides, we observed increases in LDH level in the placenta, fetus tissues, and maternal blood plasma. Unexpectedly, the severity of changes in biochemical parameters are less pronounced in rats treated with saffron and exposed to low dose gamma radiation. It could be concluded that saffron at a certain stage and certain doses could exert considerable toxicity symbolized serious life threats during pregnancy and low dose gamma irradiation could oppose this harmful action.

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