Aflatoxin in roughage, concentrates and compound feed from dairy farms located in Erzurum province, and the presence of Aflatoxin M1 (AFLM1) in the milk of animals fed with these feeds were determined in four different seasons. The mean level of Aflatoxin M1 detected in milk samples was 0.03 ppb. Aflatoxin M1 levels in the milk samples taken from the holdings were lower in autumn and summer (0.02 ppb) compared to winter and spring (0.04 ppb). The total aflatoxin levels in feed samples were higher in spring and summer, compared to autumn and winter. Furthermore, Aflatoxin B1 (AFLB1) levels in roughage, concentrate and compound feed were significantly higher in spring and summer, compared to autumn and winter (P<0.01). The results obtained in this study demonstrated that Aflatoxin B1 received with compound feed by cattle led to a carry-over of Aflatoxin M1 into milk at a level of 1.219±0.4139%. The mycotoxin levels detected were below the maximum residue limits laid down in the Turkish Food Codex.