Introduction: Polymorphisms within miRNAs binding sites are associated with miRNAs function. The aim of this study was to investigate the relationship between rs61764370 polymorphism within let-7 miRNA binding site in KRAS gene and the risk of lung cancer in Iranian population. Methods: This case-control study was conducted with 100 lung cancer patients and 100 healthy persons. The rs61764370 polymorphism was analyzed using PCR-RFLP technique and direct sequencing. Results: We found a significant relationship between rs61764370 (T / G) polymorphism and lung cancer risk, the GT gen- otype (OR: 6.25; 95% CI = 2.605-15.00; P= 0.000) and G allele (OR: 5.25; 95% CI = 2.259–12.208; P= 0.000) were signifi- cantly associated with an increased risk of lung cancer. Conclusion: According to our findings, there is a significant relationship between the KRAS rs61764370 polymorphism and lung cancer risk in Iranian population and this polymorphism may be used as a marker in detection of lung cancer in the future. Keywords: Polymorphism; KRAS; lung cancer; Let-7.
Introduction: Recent studies have indicated the function of polymorphisms in the miRNAs-binding sites within the target genes in cancer incidence. The aim of this study was to investigate the relationship between KRAS rs712 polymorphism within let-7 microRNA-binding site and lung cancer (LC) risk in the Iranian population. Materials and Methods: This case-control study was performed among 100 patients with LC and 100 healthy individuals. The rs712 polymorphism in KRAS gene promoter was evaluated using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and direct sequencing. Statistical analysis was performed using SPSS software and t-test. Results: The findings of this study revealed that rs712, TT and GT genotypes are significantly associated with LC incidence in the Iranian population (adjusted odds ratio [OR] = 3.556, 95% CI = 1.346-9.379, P = 0.01) and (OR = 2.45, 95% CI = 1.274-4.719, P = 0.01), and rs712 T allele was associated with LC incidence (OR = 1.711, 95% CI = 1.147-2.553, P = 0.01). In addition, our analysis showed an association between the rs712 TT genotype and LC metastasis (P = 0.006). Conclusions: According to our findings, rs712 T allele is significantly associated with the initiation and progression of LC in the Iranian population and it may be served as a LC screening marker in the future.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.