The aim of this study is identification and comparative analysis of bioactive phytochemicals present in methanol and ethyl acetate extracts of different plant organs and callus of Leptadenia reticulata by GC-MS technique. Methodology: The shade dried plant samples (leaves stem and root) and callus produced from leaf of L. reticulata were powdered and then sequentially extracted in methanol and ethyl acetate solvents. Total eight extracts were prepared which were Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MSE), Methanolic Root Extract (MRE), Methanolic Callus Extract (MCE), Ethyl Acetate Leaf Extract (EALE), Ethyl Acetate Root Extract (EARE), Ethyl Acetate Stem Extract (EASE) and Ethyl Acetate Callus Extract (EACE). Then, each of the extracts was further subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Results: The GC-MS analysis of the eight extracts revealed the presence of 77 different types of high and low molecular weight phytochemicals and bioactive compounds in varying quantities. Some of the phytochemicals detected for first time in L. reticulata are γ-sitosterol, Campesterol, Pristane, Hexahydrofarnesol, Stearic acid, Arachidic acid, Coniferyl alcohol, n-Tetracosanol-1, Ascorbic acid 2,6-dihexadecanoate, (2S,3S)-3,7,4'-Trihydroxy-5-methoxy-6-methylflavanone etc. These chemical compounds are considered biologically active and pharmacologically important. Conclusion: This study gives a detailed comparison of detection and identification of various bioactive phytochemicals from different plant parts (leaves, stem and root) and callus of L. reticulata. This provides a basis for the biological and biochemical characterization of some newly detected biologically and pharmacologically important phytochemical components from this plant.