The study aimed at examining the effect of reciprocal teaching strategy on students’ academic achievement in the subject of general science at elementary level. The objectives of the study were; (i) to investigate the effect of using reciprocal teaching strategy on academic achievement of 5th grade students, (ii) To investigate the effect of reciprocal teaching strategy on high achiever and low achiever of 5th grade, (iii) To observe the participation of the students in reciprocal teaching strategy. Sample of the study was consisting of 40 students studying general science in class 5th. A Pre-test Post- test equivalent group design was used to conduct the study. For collection of data teachers made test and observation sheets were developed. Statistical techniques such t-test, percentage, S.D and mean were used to analyze the collected data. The study is equally significant for students, teachers, curriculum developers and future researchers. It was found that the participation of students was significant in reciprocal teaching strategy during treatment. The participation of students in reciprocal teaching strategy reported an increasing trend after the first week to week 6.With the help of reciprocal teaching strategy students readiness to learn, their attention to reading and listening, their ability to generate questions from the text, their activeness during the discussion and their confidence about sharing their ideas in a group increased hundred percent. Reciprocal teaching had great effect on student’s academic achievement.

Poultry meat is considered a favourite meal in Pakistan. It is one of the essential constituents of the human diet daily because it contains protein, vitamins, minerals, and energy. Poultry meat can cause illnesses like food poisoning and typhoid etc. Our study aims to isolate pathogenic microbes and nutritional analysis of different poultry meat samples. Samples of domestic (30) and broilers chicken’s (30) meat were collected from different areas (Swat, Malakand, Mardan, Charsadda and Peshawar) of the market randomly. Selective and differential media were used for bacterial growth. The staining techniques and biochemical test were used to identify the bacteria as gram-negative or positive. Different biochemical tests, like Oxidase, Catalase and Triple Sugar Iron (TSI), were performed for the bacterial identification. Disc diffusion procedure was performed to test a panel of antibiotics belongs to different classes against Gram-positive and Gram-negative bacteria.  The nutritional analysis was performed by AOAC method. Salmonella, Escherichia coli, Staphylococcus aureus, Clostridium perferingens, Listiria monocytogenes, Yersinia enterocolitica and Campylobacter jejoni were seven different types of bacteria identified from meat sample. Aspergillus and Penicillium, fungi were identified from broiler chicken meat. From the result of antibiotic sensitivity, the most potent antibiotics found against bacteria were ciprofloxacin and Cefotaxime (54%) respectively, followed by ceftriaxone and Gentamicin (40%), respectively, followed by ceftazidim and Imepenem (19%) while pepracillin and penicillin antibiotic were mostly showed resistant. The result of Nutritional analysis showed that crude protein, crude fats, crude fibers, crude ash and crude moisture in domestic chicken meat ranged from 84.19 to 90.52%, 0.62% to 2.30%, 0.36% to 0.65%, 3.70% to 93% and 1.9% to 3.77% respectively. Similarly, in Broiler chicken meat, the percentage of crude protein, crude fats, crude fibers, crude ash and crude moisture ranged from 75.35% to 86.10%, 4.12% to 7.46%, 0.52% to 0.95%, 4.20% to 6.25%, and 1.92% to 2.78% respectively. The study concludes that domestic chicken meat contained a higher percentage of crude protein and crude moisture than broiler meat while a lower percentage of crude fats, crude fiber and crude ash than those of commercial broilers.

Following the announcement of the pandemic of COVID-19 in December 2019, several studies focused on how to early predict the severity of the disease in symptomatic and asymptomatic patients. Many cytokines including interleukin-6, interleukin-8, and tumor necrotic factors have been concluded as strong indicators for COVID-19 infection. Additionally, miRNAs have been associated with dysregulation in the immune system. The aim of this study are the following: (1) to estimate the level of miRNA-16–2-3P, miRNA-618, IL-8, IL-1β as predictors for SARS-CoV-2 complications in PCR negative and positive patients; (2) to assess the biological role and effect of these miRNAs on SARS-CoV-2 pathogenicity. Our study showed that the level of IL-1β had been significantly associated with patient who need hospitalization, also the alteration of the level of miRNA-16–2-3P, miRNA-618 is positively correlated with the admission of these patients and influence the outcomes of SARS-cov-2 infection. Measurement of miRNA-16–2-3P, miRNA-618, IL-1β could be a good predictor of COVID-19 patient outcome. However the measurement of IL-8 levels during immune responses in the admitted and in ICU patients could have a prognostic value.

Milk comprises proteins, fats, minerals, carbohydrates, multiple vitamins, and various special substances dispersed in water. Different types of pathogenic microbes present in unpasteurized raw milk, cause gastrointestinal tract infections and food-borne infections. The current study was based on evaluating pathogenic microbe’s frequencies among the mammal (buffalo, cow, camel, goat and sheep) milk and assessing their nutritional parameters along with the screening of antibiogram of these pathogens in the district Peshawar, Pakistan. A total of 25 milk samples (05 from each mammals) were collected. Among the analyzed milk samples, S. aureus and Shigella spp. were observed n= 04 (16%), while E. coli, Salmonella spp., and P. aeruginosa were n= 03 (12%). The S. aureus was found highly resistant (100%) to Meropenem, Gentamicin, Amoxicillin, Aztreonam, and Doxycycline, while found highly sensitive (50%) to Amikacin, and Levofloxacin. E. coli showed high resistant (100%) to Meropenem, Ciprofloxacin, Amoxicillin, Ampicillin, and Doxycycline, while found to be highly sensitive (50%) to Levofloxacin, and Cefotaxime. The Salmonella spp. was found highly resistant (100%) to Amikacin, Meropenem, Amoxicillin, Cefotaxime, Ampicillin, Aztreonam, and Doxycycline, while found highly sensitive (50%) to Ciprofloxacin, and Gentamicin. The Shigella spp. was found highly resistant (100%) to Meropenem, Amoxicillin, Ampicillin, and Doxycycline, while found highly sensitive (25%) to Amikacin, Levofloxacin, Ciprofloxacin, Gentamicin, Cefotaxime, and Aztreonam. The P. aeruginosa was highly resistant (100%) to Amikacin, Levofloxacin, Ciprofloxacin, Gentamicin, Cefotaxime, Amoxicillin, Aztreonam, Doxycycline, while found highly sensitive (25%) to Meropenem, and Ampicillin. The high ESBL-producing bacterial pathogen was found E. coli (66.66%), followed by Shigella spp. (50%), Salmonella spp., and P. aeruginosa (33.33%), respectively. The nutritional analysis of mammal milk samples significantly (p < 0.05) with different parameters. Among these parameters, the fat Mean ± SD (3.36 ± 0.11), protein content Mean ± SD (2.91 ± 0.04), lactose content Mean ± SD (4.31 ± 0.10), and total solid content Mean ± SD (11.61 ± 0.27) were observed. In conclusion, of the current study, pathogenic microbes are present in unpasteurized milk, which shows high resistance to various antibiotics, and the nutritional values of milk were also low.

The rapid growth of urbanization and industrialization are regarded as the main source of environmental pollution, particularly aquatic pollution. This study was conducted to evaluate the pollution load of industrial effluents (IE) and to utilize algal species Cladophora glomerata (CG) and Vaucheria debaryana (VD) as cost effective and environmentally friendly bioremediators for the heavy metal (HM) contaminated IE. Samples of IE were collected from the main sewage line of Hayatabad Industrial Estate (HIE) and analyzed for physicochemical parameters using standard methods and HM concentration by Atomic Absorption spectrophotometry. The load of electrical conductivity (EC), dissolved oxygen (DO), biological oxygen demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), cadmium (Cd) and lead (Pb) were found 5.03 mS/cm, 186 mg/L, 168 mg/L, 336 mg/L, 46.30 mg/L, 53.72 mg/L, 0.507 mg/L and 1.211 mg/L, respectively. The IE was then treated with algal species collected from Peshawar and were transplanted in four pots for 14 days where pots labeled as CTVD and CTCG were serving as control having tap water and VD and CG containers as treatment pots provided with IE. Results found a considerable decrease in EC (49.10–81.46%), DO (3.76-8.60%), BOD (7.81–39.28%), COD (7.81–39.28%), TSS (38.09-62.21%) and TDS (38.09–62.21%), Cd (41.02-48.75%) and Pb (48.72-57.03%). The One-Way ANOVA and T-test analysis revealed that HM concentrations were significantly (p≤ 0.05) reduced by the phycoremediation. The analysis found that C. glomerata removed 48.75% of Cd and 57.027% Pb from IE. The study concluded that phycoremediation using C. glomerata and V. debaryana is one of the environmentally friendly techniques. The procedure is cost-effective and can be utilized for the remediation of IE due to the algal efficiency in terms of HMs removal.