When the metabolically obtained 3sS-labelled sheep pituitary prolaetin-rich fraction was subjected to chemical deglycosylation the radioactivity was retained in the immunoprecipitable prolactin. 35S-labelled prolactin-fich pituitary extract was fractionated on SDS-PAGE and protein was extracted from prolactin positive bands. When the extracted 3sS-labelled prolactin was hydrolysed by alkali and then chromatographed on a thin layer of silica, it showed the presence of a radioactive compound which had an Rf value identical to the standard Tyr-O-SO 4 synthesized and characterized in our laboratory.

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