Plumbago zeylanica is a pharmaceutically important plant which belongs to Plumbaginaceae family. It possesses wide range of pharmacological activities which includes antibacterial central nervous system stimulatory activity, anti-fungal, anti-inflammatory, anti-hyperglycemic, anti-cancer and anti-atherosclerotic activity. Fatty acid methyl ester analysis reveals the presence of various fatty acids in the plant. The present study deals with Fatty acid methyl ester profile of five different accession of Plumbago zeylanica. Results reveal that these accessions are rich in Octadecadienoic acid (8-22%), Octadecatrienoic acid (7-24%) and Pentadecanoic acid (11-22%) fatty acids. Accession number 421418 is contains the highest amount of Pentadecanoic acid and 9,12,15 Octadecatrienoic acid. Accession number 439212 contains highest amount of Hexadecanoic acid. Highest amount of 9,12 Octadecadienoic acid was present in accession number 524441.