This research focuses on types of substrate materials (rice husk, coconut fiber, sand), the rate of media mix between coconut fiber, rice, and concentrations of Thiamine HCl (vitamin B1) on the growth of Rainbow Vinegar (Beta vulgaris subsp. Vulgaris). In the studies of nutritional absorption and metal toxicity in the roof, it is essential to growing plants without technical damage. The results showed that Hoagland solution combined coconut fiber, sand, and Thiamine HCl (vitamin B1) suitable for Beta vulgaris subsp. Vulgaris. The Rainbow grows remarkably from 20 days to 30 days for a faster, cleaner, and cultivating soil environment. The method presented here provides. Vulgaris to obtain a healthy plant having a well-developed root system with many lateral roots.