No abstract

Lipase-catalyzed esterification of glucose with fatty acids in ionic liquids (ILs) mixture was investigated by using supersaturated glucose solution. The effect of ILs mixture ratio, substrate ratio, lipase content, and temperature on the activity and stability of lipase was also studied. The highest yield of sugar ester was obtained in a mixture of 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TfO]) and 1-methyl-3-octylimidazolium bis[(trifluoromethyl)-sulfonyl]amide ([Omim][Tf(2)N]) with a volume ratio of 9:1, while Novozym 435 (Candida antarctica type B lipase immobilized on acrylic resin) showed the optimal stability and activity in a mixture of [Bmim][TfO] and [Omim][Tf(2)N] with a 1:1 volume ratio. Reuse of lipase and ILs was successfully carried out at the optimized reaction conditions. After 5 times reuse of Novozym 435 and ILs, 78% of initial activity was remained.

For the enhancement of enzyme activity, application of ultrasound irradiation on lipase-catalyzed esterification of fructose with palmitic acid in ionic liquids (ILs) mixture containing supersaturated fructose solution was investigated. In the mixture of [Bmim][TfO] and [Omim][Tf 2 N] (1:1, v/v), 1.44 times higher enzyme activity (29.2 µmoL/min/g) was achieved under ultrasound irradiation. Besides, ultrasound irradiation enhanced enzyme stability in viscous ILs mixture. After 5 times reuse of Novozym 435 and ILs mixture, 84.4% of initial enzyme activity was remained under ultrasound irradiation, while the residual activity using magnetic stirring only method was 76.2%. These results show that enzymatic reaction in viscous ILs mixture under ultrasound irradiation is an effective method for enzyme activity, as well as, enzyme stability resulting in economic competitiveness of green process. © KSBB hÉóïçêÇëW=áçåáÅ=äáèìáÇëI=ÑêìÅíçëÉ=é~äãáí~íÉI=ï~íÉê=ÅçåíÉåíI=ìäíê~ëçìåÇ=áêê~Çá~íáçå= = = = =

Nghiên cứu mô tả đặc điểm lâm sàng, cận lâm sàng và phân tích kết quả điều trị trẻ mắc hội chứng thận hư tiên phát kháng Corticosteroid. Nhóm tác giả thực hiện mô tả tiến cứu trên 37 trẻ mắc hội chứng thận hư tiên phát kháng Corticosteroid ở trẻ em tại Bệnh viện Sản Nhi Bắc Ninh từ tháng 01/2021- 12/2021. Kết quả nghiên cứu có 27/37 (73%) nam, 10/37 (27%) nữ, tỷ lệ nam/nữ: 2,7/1, tuổi trung bình 10,1±3,3 tuổi. Lâm sàng: 54,1% (20/37) hội chứng thận hư thể đơn thuần, 94,6% (35/37) hội chứng thận hư tiên phát, 100% có phù ở các mức độ, trong đó 27,1% (10/37) phù nặng, 6/37 (16,2%) tăng huyết áp. Cận lâm sàng: 28/37 (75,7%) tăng số lượng bạch cầu, 100% giảm albumin máu, 36/37 (97,3%) giảm protein máu, 02/37 (5,4%) thiếu máu và tăng ure máu, 01/37 (2,7%) tăng creatinin mức độ nhẹ. Kết quả điều trị mức độ phù giảm dần qua thời gian điều trị, sau 6 tháng, 12 tháng tỷ lệ không phù đã tăng nhanh (đều trên 90%). Thời gian trung bình protein niệu trở về âm tính là 1,76 ± 0,99 tháng. Có sự giảm dần số lượng bạch cầu trở về giới hạn bình thường qua thời gian điều trị và có sự giảm lượng urea máu có ý nghĩa thời điểm sau 12 tháng điều trị so với sau 6 tháng (p<0,05), tỷ lệ kết quả điều trị chung 21/37 (56,8%) tốt. Biểu hiện lâm sàng, cận lâm sàng của bệnh nhi mắc hội chứng thận hư kháng Corticosteroid nặng nề, kết quả điều trị chung chưa cao.

Cardiomyopathies (CMs) are a heterogenous group of disorders that affects the heart muscle. Incardiomyopathies, phenotypic overlapping among the inherited cardiovascular diseases (CVDs) limits theability to establish a diagnosis based solely on clinical features. Here, we developed a next generationsequencing (NGS) assay to analyze a panel of 142 known cardiomyopathy genes in 9 Vietnamese patientsfrom Children Hospital 2, Hochiminh City and Medical University Hospital, Hochiminh City, Vietnam.Whole exome sequencing (WES) - a technique which determines the variations of all coding regions (exons)of the known genes - validated a total of 65 rare variants in 18 cardiomyopathy genes among the studiedVietnamese unrelated patients. Of 65 variants identified, 28 variants were homozygous and the other 37 oneswere heterozygous. Among the 65 variants, TTN gene variants accounted the most for 13 mutations, which areknown to be benign. Other groups of 9 and 8 mutations belong to SYNE1 and MYPN genes, respectively. Tenout of 65 mutations distributed equally to NDUFV2 and SCN5A gene variants. We detected 6 and 4 variantsfor SYNE2 and COX15 genes, respectively. Each gene of DMD, KCNE1, NEBL and RBM20 has 2 variants. Asingle variant was detected for AKAP9, CAV3, DSC2, DSG2, DSP, MYBPC3 and MYH6 genes. Especially,among them, we found a novel heterozygous nonsynonymous mutation c.1527C>G on the MYPN gene. Thesegenetic results support the “pan-cardiomyopathy panel” approach, by which the molecular diagnosis ofcardiomyopathies, early identification of arrhythmia development and better clinical management ofcardiomyopathic patients are applied.