Kancra is one of the endemic fishes in Indonesia, however, its population keeps decreasing due to overexploitation. One of the ways methods to solve this problem is by implementing cryopreservation. Cryoprotectant has effect on successful cryopreservation. The purpose of this study was to evaluate the effect of free-range chicken egg yolk as a natural cryoprotectant combined with 10% of methanol to motility of spermatozoa that had been used after 48 hours cryopreservation. The egg yolk concentration used were 0%, 5%, 10%, 15%, 20%, and 25%. The sperm was collected by stripping and diluted with methanol and fish ringer, then it was equilibrated at 5 °C for 10 minutes. After that, it was frozen at -10°C for 48 hours and thawed at 40 °C for 1 minute. The spermatozoa motility was analyze using ANOVA and Tukey test. The results show there is a significant effect (p <0.05) on all of egg yolk concentrations. The 5% concentration of free-range chicken egg yolk shows the highest motility percentage of 84.06 ± 1.67%.

Effect of egg yolk of free-range chicken and methanol as a cryoprotective agent for the sperm preservation of cyprinid fish, Neolissochilus soroides (Valenciennes, 1842