Phytochemical study on the stem bark of P. arborescens has isolated six secondary metabolites from the crude extracts by using column chromatography, while identification was performed by using Gas Chromatography - Mass Spectrometry (GC-MS), Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared (FTIR). The isolated metabolites were identified as caryophyllene oxide (1), α-bisabolol (2), benzamide 2-(methylamino) (3), 2-ethylpiperidine (4), piperine (5) and methyl eugenol (6). Toxicity test on the four crude extracts of P. arborescens shows a high cytotoxicity against Artemia salina brine shrimp with LC50 values ranging from 13.12 to 58.70 µg/mL. Greater cytotoxicity of the crude extracts of P. arborescens indicated the presence of potent cytotoxic components in this Piper spp. Antioxidant assay of P. arborescens against 2-diphenyl-1-picrylhydrazyl (DPPH) indicated moderate antioxidant activities of methanol, dichloromethane, chloroform and hexane crude extracts with EC50 values of 21.68, 23.82, 32.88 and 36.88 µg/mL, respectively.

Objective: The purpose of the study is to carry out fluorescence analysis, phytochemical, in vitro antioxidant In-Vitro Thrombolytic Activity of Kabasura Kudineer Herbal Concoction. Methods: In the present study, fluorescence analysis, phytochemical, antioxidant In-Vitro Thrombolytic Activity of Kabasura Kudineer Herbal Concoction are carried out using standard procedures. Result: The fluorescence analysis under visible and ultraviolet light for Kabasura Kudineer herbal concoction treated with various chemical reagents shown different fluorescence effect. It showed a significant antioxidant activity in DPPH, Reducing Power and H2O2 scavenging methods. From our study, we also found that it showed 41.6% clot lysis activity respectively and they showed significant % of clot lysis effect with reference of Streptokinase (75.2%) and water (2.93%). Conclusion: The findings of the present study suggest that Kabasura Kudineer herbal concoction could be a potential source of natural antioxidant that could have greater importance as therapeutic agent in preventing has significant thrombolytic action. Additional studies are greatly essential for further drug development.