Background: Hyssopus officinalis L. (hyssop), a species native to the Caspian Sea region, has been cultivated in the Republic of Moldova as aromatic plant and has been used in folk medicine as antitussive, expectorant, carminative, digestive and sedative remedy. Material and methods: The aerial parts of H. officinalis L., with pink, white and blue flowers, were collected from the collection of the Scientific Practical Center in the Field of Medicinal Plants during flowering phase. The extracts were obtained with 70% ethanol by maceration with stirring. The concetration of the extracts was done with the rotative evaporator Laborota 4011. Identification of phenolic compounds in dried extracts from hyssop herb was carried out by thin-layer chromatography. The total content of hydrocyanamic acids was measured in plant products and dried ethanolic extracts, by spectrophotometric method, with Arnow’s reagent. Quantitative analysis of total phenolic content was carried out by UV-spectrophotometry analysis, using a Metertech UV/VIS SP 8001 spectrophotometer. Results: The chlorogenic (Rf 0.47) and caffeic (Rf 0.93) acids were identified in the three genotypes of Hyssop herba, with pink, white and blue flowers. The total of hydroxycinnamic acids, expressed in caffeic acid, for both, aerial parts and dry extract, was the highest in H. officinalis L. with white flowers (1.484 mg/g; 3.014 mg/g respectively), followed by H. officinalis L. with pink flowers (1.190 mg/g; 2.915 mg/g) and H. officinalis L. with blue flowers (1.015 mg/g; 2.851 mg/g). The highest polyphenol content, expressed in gallic acid equivalent (GAE), was found in the dry extract of H. officinalis L. with blue flowers (39.056 mgGAE/g dry extract). Conclusions: This study showed that the extract of the Hyssopus officinalis L. containing phenolic compounds, can be used as a natural antioxidant in pharmaceutical and cosmetic industries.

Background: Urinary tract infections are the most common urogenital diseases, with an increased incidence in men and older people. Urogenital infections are caused by Gram-negative bacteria, in which Escherichia coli predominates with a share of 80%. The evolution of microbial resistance to preparations used in curative-prophylactic institutions, induces the need of the reintroduction of nitrofurans, noteworthy for their wide spectrum of antibacterial activity. Material and methods: For the study, suppositories with furazidine were prepared by hand rolling and by melting and molding methods. Quantitative analysis was performed spectrophotometrically on a UV-VIS Perkin Elmer Lambda 40 spectrophotometer. All solvents and reagents had the degree of purity ”pure for analysis” and ”chemically pure”. Results: Duble cast method was applied to identify the exact mass of hydrophobic (cocoa butter, suppocire) and hydrophylic (polyethylene glycol mixtures) excipients. All the formulated suppositories were subjected to quality tests and showed acceptable physical characteristics and uniformity of drug contents. The UV-VIS spectrophotometric method for quantitiative determination of furazidine was developed and validated. The validation results showed that the developed method is simple, fast, accurate and robust. Conclusions: Suppositories with furazidine were prepared by classic technological methods. Preparation of the suppositories with furazidine on cocoa butter excipient is a suitable alternative for individual medicinal prescriptions. The UV-VIS spectrophotometric dosing method for furazidine in suppositories was developed and validated

Objective: Evaluation of effects of RDN on left ventricle remodeling in patients with resistant hypertension and heart failure with preserved ejection fraction. Design and method: The study enrolled 125 hypertensive patients, who after 3-week of standardized treatment with Losartan, Amlodipine and Indapamide and confirmation of their drug resistance, were randomly assigned into three groups depending on medication supplemented to previously administered: group I - selective I1-imidazoline agonist Moxonidine, group II - cardioselective beta-blocker Bisoprolol and group III - renal denervation. All patients underwent a transthoracic echocardiogram at baseline, one, two and three years follow-up. Renal denervation was performed in the main renal arteries and their branches. Results: Transthoracic echocardiography at baseline revealed LV hypertrophy in all patients. The majority of patients in all three research groups had concentric hypertrophy (52% versus 60% and 56% in group I, II and III, respectively), the normal pattern not being recorded in any group. An authentic reduction in LV mass index was noted from 1-year follow-up in all three observational groups, the progressive reduction being maintained until the end of the study, the group of patients undergoing renal denervation manifested a net superior effect to both pharmacological groups. So, at 3-years follow-up LV mass index was 128,57±4,91 g/m2 in Moxonidine group versus 126,0±5,34 g/m2 in Bisoprolol group and 94,44±3,90 g/m2 in RDN group, p < 0.001. Analyzing the impact of various therapeutic regimes on the geometric pattern of the LV, we can note the fact that both treatment schemes with SNS blockers and RDN favored LV reverse remodeling, its normal pattern being regained by 5 (10%) patients from group I and 7 (28%) patients from group III. This time as well RDN demonstrated absolute superiority over both pharmacological treatment regimens (Figure 1). Conclusions: The obtained data confirm the benefit of RDN treatment on the reverse remodeling of the LV by significantly reduction in LV mass index and inducing the recovery of its normal physiological pattern in practically a third of treated patients at 3 years post-procedurally.