In historical times, plague epidemics intermittently ravaged Europe for more than 1,400 years, and still represent a threat in many countries all over the world. A debate is ongoing about the past plague, if it killed randomly in a population or discriminated among persons on the basis of their biological features. To address questions of plague lethality, we reviewed a large number of anthropological studies published in the last twenty years on victims of the past pestilences in Europe. In particular, we focused on data concerning demography (age at death and sex determination), and health status (skeletal biomarkers). We applied to these data a model system based on Multiple Linear Regression, which aimed to discern among possible predictors of sex-selective plague lethality in entire populations, in different periods and regions. Based on available data, we lack evidence for general trends of association between biological features. Differences in sex ratio are more likely due to the original population compositions or to distinct cultural behaviours of the two genders. We concluded that generalizations on biological evidence are not feasible for ancient plagues if we exclude that the infection possibly killed primarily persons between 5–10 and 20–35 years of age.

Objective Frailty is the physiological stress that individuals suffer during their life. In past populations, frailty is conventionally assessed through the occurrence of different biomarkers of biological stress. Some efforts have been made to propose indexes that combine all biomarkers. However, these indices have some critical limitations: they cannot be used on incomplete skeletons, do not consider the severity and/or healing of lesions, and assign equal importance to different biomarkers. To address these limitations, we propose a new index to assess frailty in skeletal individuals. Material and Methods By statistically analyzing a large amount of osteological data available from the Museum of London, and using a Logit model, we were able to define a different weight for each reported biomarker of frailty, based on their importance in increasing the risk of premature death for the individuals. Results The biological index of frailty (BIF) is the weighted mean of all biomarkers scored on the individuals, according to a different degree of importance assigned to each one. It also considers the severity and healing of the biomarkers when this is relevant to diagnose frailty. We applied BIF on a sample of Monastics and Non‐Monastics from medieval England and compared it with the skeletal index of frailty (SFI). Discussion BIF is the first frailty index that gives a different weight to each skeletal biomarker of stress, considers both severity and healing of the lesions, and can be applied on partial skeletal remains. The comparison with SFI showed that BIF is applicable to a larger number of skeletal individuals, revealing new differences between the Monastic and the Non‐Monastic groups.

Intra vitam porous lesions of the skull (Cribra Orbitalia and Porotic Hyperostosis) are pathological conditions due to 28 genetic or acquired chronic anemia. They are the most reported skeletal lesions in human skeletal remains and are 29 routinely used to assess health, hygiene and nutritional status of past populations. Despite the existence of a number of 30 proposed classifications, there is no generally accepted classification system used by all, with clear advantages over the 31 others. 32Here, we propose a new evaluation form (BoPLE-Bone Porous Lesion Evaluation) that takes in consideration all the 33 observable features of bone porous lesions, integrating existing qualitative criteria for the evaluation of severity and 34 healing's conditions with a new quantitative analysis based on the count of pores/cm2. Porotic Hyperostosis and Cribra 35Orbitalia were investigated using the newly developed evaluation forms on 189 cranial bones from several distinct 36 archaeological sites. Reliability and reproducibility of both existing qualitative scoring criteria and the new quantitative 37 method were statistically tested. 38We believe that the new proposed classification system, which takes into consideration diverse parameters like surface 39 area of lesion, location of lesion on cranial vault, and number of pits per surface area, represents a progress in the objective 40 evaluation of porous bone lesions Its use will allow the determination of the severity of the lesion, thus provide data to 41 assess conditions of frailty in past populations.

During pre-adolescence, several variables connected to growth and weight status could influence physical performance and technical skills. This study aims to assess the influence of growth-related anthropometric changes, weight status, and basketball experience on physical performance in pre-adolescent basketball players. The sample consisted of 50 male basketball players (12.2 ± 0.4 years-old) included in the U-13 category. Anthropometric characteristics were collected using standard procedures. Physical performance was evaluated by 20 m Dash, T-test, squat jump, countermovement jump, and medicine ball throw. In order to assess growth-related changes, we conducted two surveys within a 10-week time span. Student’s t-test was used to compare the data collected in the two surveys and multiple regression analyses were performed to assess the effects of growth, weight status, and basketball experience on performance. After 10 weeks there was a significant increase in FFM (Fat-Free Mass) and a significant decrease in fat parameters. Moreover, between the two surveys, all motor tests significantly improved. The results of linear regression models showed that changes in %F (percentage of body fat) were significantly associated with speed and agility, while handgrip strength and weight status were associated with upper limb explosive strength. Basketball experience was a significant predictor of all three motor tests. In conclusion, body composition changes, years of experience, and weight status influenced physical performance and players’ motor skills ability, especially speed, agility, and upper limb explosive strength.

The plague of 1630–1632 was one of the deadliest plague epidemics to ever hit Northern Italy, and for many of the affected regions, it was also the last. While accounts on plague during the early 1630s in Florence and Milan are frequent, much less is known about the city of Imola. We analyzed the full skeletal assemblage of four mass graves (n = 133 individuals) at the Lazaretto dell’Osservanza, which date back to the outbreak of 1630–1632 in Imola and evaluated our results by integrating new archival sources. The skeletons showed little evidence of physical trauma and were covered by multiple layers of lime, which is characteristic for epidemic mass mortality sites. We screened 15 teeth for Yersinia pestis aDNA and were able to confirm the presence of plague in Imola via metagenomic analysis. Additionally, we studied a contemporaneous register, in which a friar recorded patient outcomes at the lazaretto during the last year of the epidemic. Our multidisciplinary approach combining historical, osteological and genomic data provided a unique opportunity to reconstruct an in-depth picture of the last plague of Imola through the city's main lazaretto.