Twenty one bioactive phytochemical compound were identified in the methanolic extract of Cyperus aucheri Jaub. The identification of phytochemical compounds is based on the peak area, retention time, molecular weight and MS Fragmentation. GC-MS analysis of Cyperus aucheri Jaub. Revealed the existence of the 3-Phenylheptanoic acid, benzylammonium salt, 2-(benzyloxymethyl)-5-methylfuran, 4-methoxycarbonylphenyl)methyl 4-methylbenzoate, Benzoic acid, 4-methyl-,[4-(methoxycarbonyl)phenyl]methyl ester, dodeca-5,7-diyne-1,12-diol, 4-Isopropyl-3,7-dimethyloctahydro-1H-cyclopenta[1,3]cyclopropa[1,2]benzol-3-ol, Benzoic acid,4-methyl-,[4- (methoxycarbonyl)phenyl]methyl ester, 6-Oxo-heptanoic acid methyl ester, Benzamide, N-[4-(3-furan-2-ylacryloyl)phenyl]-4-methyl-ester, O-alpha-dextro-glucopyranosyl-(1-3)-beta-dextro-fructofuranosyl-alpha-dextro-glucopyranoside, 1-methyl-5-(propan-2-yl)-3,6,7-trioxatricyclo[3.2.2.02,4]nonane, 2,3-diacetyloxypropyl (9E,12E,15E)-octadeca-9,12,15-trienoate, 2,3-bis(acetyloxy)propyl ester, Methyl 8-oxohexadecanoate, ethyl (3α,5β,7α,12α)-3,7,12-trihydroxycholan-24-oate, Furan-2-carbohydrazide, N2-(3-indolylmethylene), 1,2-Propanediol, 3-(octadecyloxy)-,diacetate, N,N-Dimethyltetradecylamine, 1-O-butyl 2-O-tetradecyl benzene-1,2-dicarboxylate, 2,7-Diphenyl-1,6-dioxopyridazino[4,5:2’,3’]pyridazin, 1-Heptadecanecarboxylic acid, Dasycarpidan -1-methanol, acetate ( ester). It contain chemical constitutions which may be useful for various herbal formulation as Antioxidant, Anticancer, Antigenotoxic activity, Antimicrobial Activity, anti –inflammatory, Anthelmintic, Nematicide, Antihistaminic, Antieczemic, Insectifuge, Treat asthma, Stomach ach, Arthritis, dysentery, Malaria, nervous disease, Antitumor, Antidiarrheal, antifungal activity.