BackgroundIt is necessary to know the in vitro behavior of different attachment systems to be used clinically. The evolution of retention capacity over 10 years (14,600 insertion/de-insertion cycles) was determined in vitro, evaluating two overdenture attachment systems (Locator® and OT Equator®).Material and MethodsThe study used an implant replica compatible with the abutments of both systems. 10 Locator® and 10 OT Equator® attachments were screwed to the abutments. Nylon inserts were attached and tested, subjecting them to 14,600 insertion and de-insertion cycles (representing 10 years functional life) in axial direction. The universal test machine crosshead speed was 50 mm/min with a de-insertion range of 2 mm.ResultsThe initial retention of Locator® was 17.02 N and of Equator® 16.36 N. After 14,600 cycles, Locator® suffered a mean loss of retention of 50.89%, while Equator® lost 69.28%. Both systems showed retention increases up to the first 1,000 cycles, which decreased thereafter up to 14.600 cycles. Statistically significant differences between the systems were found after 7,500 cycles.ConclusionsBoth systems presented acceptable retention capacities after 14,600 cycles. Significant differences in retention force between the systems evolved after 7,500 cycles (5 years in vitro use). These results should be treated with caution and should be verified clinically. Key words:Denture, mandibular prosthesis implantation, attachment, dental implant-abutment connection, denture retention.