Antimicrobial resistance is majorly an issue of public health concern. The aim of this study is to isolate and identify Escherichia coli from samples of stool and urine obtained from the clinical settings at Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, and to determine their antibiotics susceptibility patterns. Nineteen (19) of stools and 22 of urine samples were analysed using standard microbiological and biochemical techniques and 11 pure isolates were obtained comprised of 5 (12,2%) isolates from urine and 6 (14,6%) isolates from stool. Antibiotics susceptibility studies were conducted using Kirby and Bauer disc diffusion method, and the results were determined using the Clinical and Laboratory Standards Institute (CLSI) guides.The studies showed that all the E. coli isolated were 100% resistant to augmentin, gentamycin, streptomycin, tetracyclin and chloramphenicol, and 90.90% resistant to oflaxin, sparfloxacin, and amoxycillin while the isolates were susceptible tociprofloxacin (26.33%), and pefloxacin (45.46%).Effective hygiene must be encouraged and indiscriminate usage of antibiotic must be avoided in the study area.