Conflicts of Interest and Source of Funding: No conflicting interests are declared.

No abstract

The relationship between aflatoxins and liver cancer is well stablished. In addition the inhalation exposure to carcinogen aflatoxin B1 (AFB 1) is considerable. Genotoxic chemical is known to react with DNA either directly of after metabolic activation to form adducts, a step thought to be relevant with respect to chemical carcinogenesis. The presence and the amount of specific DNA adducts provide a good indication of chemical exposure and genetic damage resulting the exposure to carcinogens and account for same of factors affecting individual sisceptibility to cáncer. Analysis de DNA addfucts requires that the sensivity of the methods to be sufficients hight to allow detection of about 1 adduct / 109 normal nuceotides.

Ruta graveolens L. and Ruta chalepensis L. are plants used in folkloric medicine as antispasmodics, digestive and for intestinal gases. Animals used as experimental model were rates of the Wistar line, adult females, clinically healthy and with a weight average of 250 grams. We used strips of stomach and duodenum. Each one of the segments mounted on two stirrups in a bath of organ isolated with Ringer-lactate solution, at 37° C , pH: 7.3- 7.4, and bubbled with 95% O2, 5% CO2. One of the stirrups was connected vertically to the bottom of the bath and the other to a transducer of tension connected to a Beckman polygraph. We applied 500 mgs of basal tension. After the stabilization, the ethanolic extract of Ruta ssp was added in increasing doses.At 50?l/ml the tone lower 23% in small intestine and lower 27% in stomach. However at 100 ?l/ml the tone lower 32% and 35% respectively. In the other parameters the amplitude decrease 50% in the stomach at dose of 5 ?l/ml while in the small intestine the amplitude lower 60%. With 10 ?l/ml the amplitude change in both organs ( 96% in small intestine, and 75% in stomach).The frequency changes in both organs ( 32% in small intestine, and 50% in stomach) at 10 ?l/ml Rue showed decreased effects on isolated small intestine and stomach were is dose dependent, maybe we were demonstrated the effects digestive of Ruta.