Introduction: Blood Transfusion is the integral part of modern medicine. Aim of the study was to assess the changing trends in the prevalence of transfusion transmissible infections in blood donors during last 13 years. Material and Methods: A retrospective study was conducted at blood bank of Shri Vasantrao Naik Government Medical College Yavatmal, Maharashta. A total of 111462 donors were screened over a period of thirteen years. Results: A declining trend was observed in all transfusion associated infections among the donors. The overall seropositivity of HIV, HBV, HCV, VDRL and Malaria was found to be 0.46%, 1.27%, 0.06%, 0.12%, 00% respectively. Conclusion: Prevalence of HIV infection is decreasing in the blood donors as a result of drastic measures to combat the disease in general population.The HBV infection still remains a menace to be tackled, HCV, Syphlis and malaria maintain a low rate of positivity in rural India.