Binahong leaves (Anredera cordifolia [Ten] Steenis) belonging to the Basellaceae family contain flavonoid compounds, saponins, and polyphenolic alkaloids. The largest secondary metabolites of phenolic compounds are flavonoids which have pharmacological effects such as antioxidants. This study aims to determine the flavonoid content of binahong leaves (Anredera cordifolia [Ten] Steenis) from Pelem village in 70% ethanol solvent. This study uses a pure experimental method. The extract was obtained by maceration method with 70% ethanol as solvent. The maceration process was carried out for 3 days with repeated maceration 2 times and occasionally shaking. After the viscous extract was obtained, qualitative tests were carried out for flavonoid screening and determination of flavonoid content using UV-Vis spectrophotometry. The qualitative test results of 70% ethanol extract proved to contain flavonoids. Based on the results of this study, it can be concluded that the determination of total flavonoid levels using the UV-Vis spectrophotometer method was obtained at 0.1842% (w/w).

Kepok banana (Musa paradisiaca L.) is one of the most commonly consumed bananas in Indonesia. Apart from the fruit, the other potentially used is banana peel. Kepok banana peel is rich in flavonoids which has function as antioxidants that protect skin from free radicals. In this study, we optimized and conducted physical characterization for banana peels peel-off gel mask. The aim for this research was to optimize the peel-off gel mask and analized the physical characteristics of peel-off mask gel. The formulation was optimized with different concentration of active substance. The physical characterization was organoleptic observation, homogeneity, pH, dispersibility, adhesion, drying time, irritation and stability assay. Organoleptics observation showed that peel-off gel showed distinctive scent, brownish color and gel-like structure. All preparation were homogenous, pH value dispersibility, adhesion, and drying time was within the required criteria. The peel-off was not irritant as showed from irritation assay. The preparation was also stable during 12 weeks storage under room temperature condition. We conclude that banana peels peel-off gel mask was successfully formulated. Further observation for its effectivity will be required.

Bromelin memiliki aktivitas sebagai anti edematosa, antitrombotik, dan antiinflamasi pada berbagai penelitian in vitro dan in vivo. Ekstrak kasar bromelin kulit nanas didapatkan dari proses isolasi enzim kulit nanas segar yang dilakukan dengan cara sentrifugasi. Ekstrak kasar bromelin kulit nanas dikembangkan menjadi sediaan gel yang ditujukan sebagai anti edematosa atau anti memar dengan melihat profil hematologi pada tikus yang dibuat luka memar. Formulasi gel ekstrak kasar bromelin kulit nanas menggunakan bahan aktif ekstrak kasar bromelin 450 mg/kg BB dengan perbandingan konsentrasi gelling agent dan humektan yang sama besar, serta zat tambahan berupa trietanolamin, metil paraben, dan air. Tikus putih jantan dibagi menjadi empat kelompok perlakuan, yaitu kelompok I sebagai kontrol normal tanpa terapi, kelompok II sebagai kontrol positif, kelompok III sebagai kontrol negatif, dan kelompok IV sebagai kelompok sampel. Tikus dibuat luka memar dengan teknik penjatuhan beban pada punggung tikus. Parameter uji pada penelitian ini meliputi sel darah putih (white blood cells, WBC), sel darah merah (red blood cells, RBC), hematocrit (HCT), dan platelets (PLT). Berdasarkan analisis two way ANOVA, terdapat perbedaan nilai WBC yang bermakna antar waktu dan kelompok dengan nilai p sebesar 0,008 (p<0,05). Sedangkan nilai p dari RBC, HCT, dan PLT berturut-turut sebesar 0,421, 0,297 dan 0,758, yang menunjukkan tidak ada perbedaan yang bermakna. Kesimpulan pada penelitian ini adalah gel ekstrak kasar bromelin kulit nanas pada dosis 450 mg/kg BB efektif menurunkan nilai WBC pada jam ke-6 dan jam ke-12, yaitu masing-masing sebesar 13,33±2.05 (x10³/µL) dan 11.80±1.30 (x10³/µL).