Background: This study aims to discover the practice preparedness and dentists' mental condition in Semarang during Coronavirus Disease 2019 (COVID-19) pandemic. Method: This is an observational analytic study with a cross-sectional online study design. Subjects in this study is 171 members of persatuan dokter gigi Indonesia (PDGI) Semarang branch. Research subject was asked to fill questionnaire on practice preparedness during COVID-19 pandemic and depression anxiety stress scale (DASS-42) in Indonesian shared by researchers through Google form link. Data was analyzed using Spearman correlation, Mann-Whitney, and multinomial logistic regression tests. Result: Bivariate test result showed significant correlation between practice preparedness and DASS depression score (p =0.021 and r=-0.176) and anxiety DASS score (p=0.018 and r=-0.181). There was a significant correlation between training and DASS score depression (p=0.023), anxiety (p=0.025), and stress (p=0.040). Multinomial logistic regression multivariate test result depicted a significant relationship between training and moderate depression (p=0.04 and OR=5.65). Dentists with no training have a higher risk of 5.65 times on moderate depression compared to those with training during COVID-19 pandemic and infectious disease prevention and control training. Conclusion: The better practice preparedness, the milder depression and anxiety of dentists during pandemic. Practice preparedness does not affect dentists' stress during COVID-19 pandemic. Training may also have an impact on dentists' depression during COVID-19 pandemic. PENDAHULUAN Coronavirus Disease 2019 (COVID-19) adalah penyakit infeksi yang disebabkan oleh SARS-CoV-2, jenis coronavirus yang baru ditemukan dan dapat menyebabkan infeksi saluran pernafasan pada manusia. 1 Kasus COVID-19 di Indonesia pertama kali dilaporkan pada tanggal 2 Maret 2020. Jumlah kasus COVID-19 yang telah terkonfirmasi dari 510 kabupaten/kota di seluruh 34 provinsi di Indonesia pada tanggal 12 Mei 2021 sebanyak 1.728.204 kasus, terdiri atas 47.617 kasus meninggal dan 1.584.878 kasus sembuh. 2 COVID-19 menjadi kedaruratan kesehatan masyarakat serta bencana nasional yang dapat menyebabkan kematian dan berdampak pada kerugian ekonomi. 3 Pandemi COVID-19 secara signifikan dapat menimbulkan tingkat kecemasan dan stres pada tenaga kesehatan. Kekhawatiran tenaga kesehatan yang utama adalah risiko akan terlular dan manularkan infeksi, baik pada diri sendiri maupun kepada keluarga. 4 Hasil penelitian yang dilakukan pada dokter gigi di Italia menunjukkan seseorang yang memiliki tingkat ketakutan COVID-19 yang lebih rendah memiliki gejala depresi yang lebih ringan. 5