Wood apple chutney was nutritious and health beneficial product with biochemical properties such as phenolic compounds that act as antioxidant properties. The present investigation was to develop and storage study of wood apple chutney. The best quality of chutney was selected on the basis of organoleptic quality for storage study. During the storage period of chutney moisture content, pH, total soluble solid, titratable acidity, ascorbic acid and biochemical properties (total phenolic content and antioxidant activity) were analyzed. Wood apple chutney was recorded on second month that ascorbic acid, total phenolic content and antioxidant activity had 2.2 mg/100g, 143.7 mg/g and 65.4% respectively. Hence result signified that chutney prepared from wood apple fruit was contained nutritional properties that beat suitable for consumption up to 2 month after that biochemical properties start to degraded.
Wood apple is an underexploited edible fruit, that contains a myriad of phytochemicals and is used in ethnomedicine to treat a variety of diseases. This research uses Response Surface Methodology (RSM) to optimize phenolic compound extraction as significantly (p<0.01) influenced by various solvents and extraction conditions including solvent concentration (50-100%), temperature (30-60 oC) and time (2-5 hr) as independent variables, and extraction yield, phenolic content and DPPH activity as dependent variables. The optimal extraction conditions obtained were: methanol (81.46% concentration, 53oC temperature for 3.3 hr); ethanol (73.04% concentration, 45oC temperature for 3.58 hr); acetone (50% concentration, 43.48 oC temperature for 3.06 hr) and ethyl acetate (99% concentration, 49.5oC temperature for 2 hr). The maximum extraction yield (38.14%) along with DPPH activity (80.85%) were observed using methanol, and phenolic content (248.84 mg GAE/g) using acetone. The phenolic compounds (gallic, syringic, vanillic acid etc.) in optimised solvent extract were quantified using Ultra High Performance Liquid Chromatography (UHPLC-UV).
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