Potato crop is affected by numerous diseases in field, as well as in storage conditions, particularly in cold storage. Dry rot of potato is caused by Fusarium sp. Dry rot of seed tubers can reduce crop establishment by killing developing potato sprouts, and crop losses can be up to 25%, while more than 60% of tubers can be infected in storage. It is a rich source of energy, which supplement food source of a country. In this experiment, four systemic and four non-systemic fungicides were tested at different concentrations in vitro for their comparative efficacy against inhibition of the growth of Fusarium sp. through poisoned food technique. To manage these diseases effectively, different concentrations of fungicides were tested. Results revealed against Fusarium sp. that carbendazim and benomyl significantly inhibited the fungal growth even at its lower concentration (100 ppm) and proved statistically superior over the rest of the fungicides tested at different concentrations and among non-systemic fungicides, MEMC significantly inhibited the fungal growth even at its lower concentration (1000 ppm).