In this article we report the development of scaffold mimicking the cell surface topography. Using Soft lithography, in which polymer (triglyme, ethylene glycol dimethacrylate, methacrylic acid and IRGAcure 2022) is applied over the cultured cell and cured instantly using UV source. IRGA cure 2022 is a photoinitiator, which helps in solidification of the polymer when exposed to UV light source. Triglyme helps to increase the viscosity of the polymer. This method can be applied to all adherent cells. This scaffold consists of cell surface topography features. The developed scaffold can pave way in tissue engineering for various applications and cell research investigations.

Various approaches have been used to retain the dosage form in the stomach as a way ofincreasing the gastric residence time (GRT), including floatation systems; high-density systems; mucoadhesive systems; magnetic systems; unfoldable, extendible, or swellable systems; andsuperporous hydrogel systems. The aim of this study was to prepare and evaluate floatingmicrospheres of cefdinir for the prolongation of gastric residence time. Themicrospheres were prepared byCapillary Extrusion method.A full factorial design was applied to optimize the formulation. The optimum batch of microsphere exhibited smooth surfaces with good flow and packing properties, prolonged sustained drug release, remained buoyant for more than 12 hrs, high entrapment efficiency upto68%.Scanning electron microscopy confirmed the hollown structure with particle size in the order of190 μm. The studies revealed that increase in concentration of gum Karaya increased the drug release from the floating microspheres.

Gingkolide is an antiseizure medicine used as an adjuvant of partial seizures and GAD to relieve neuropathic pain. It binds to the very high affinity alpha delta site in the CNS. Although the drug's mechanism remains unclear, in genetically engineered mice and other anticonvulsive models, findings showed that it binds to alpha receptors. A rapid rise in the number of drugs added to each class of drugs has been noted. Whether in a single or multi-drug delivery form, these medications are developed into newer formulations. These newest formulations put on the market need a new investigation to estimate the medication in the formulations. In the scientific literature, the current analytical procedures for such drugs are available, but not all approaches are stable and economical to use. Few other techniques are often time-consuming. The goal of this work was to develop an RP-HPLC analytical tool for Gingkolide estimation. The drug's RP-PLC study meets the drug's optimum integrity, suitability, regeneration. The drug's LOQ and LOD were reached with elevated sensitivity. Overall, the results show that the recommended analytical approach in the formulation should be used to evaluate the drug. For regular study of the medication in its dosage form, this approach may be recommended.

The active ingredient, called piperine, is present in black pepper. The ions are very small so they are easily consumed by the tissue and nervous system, causing the chemical release within the brain. Piperine has been shown to help ease gastrointestinal ailments, help with vomiting, and has the ability to help with inflammation of the body. This explains to us how simvastatin can help expedite piperine in the body. The new, clear, effective, quick, accurate ultraviolet spectrophotometric method has to be validated and developed for the study of simvastatin and piperine in bulk and poly-herbal formulations. Data from validation experiments was tested using methodological techniques. Since processing at a wavelength of 285nm, the standard solution appeared to have a far higher absorbance than at other wavelengths. Normal simvastatin and piperine have been measured in varying amounts, and they make spectrums of overlays. In Beer Law, the concentration (C) of a solvent is plotted against the absorbance (A) from a calibration curve, as a result. A linearity range of between 14and 39μg/mL was observed. The sample was tested by prorating the standard deviation and standard error of the approximate means with the sample size, demonstrating the accuracy and the precision of the methods used in the analysis. Based on the experimental findings, it can be easily inferred that for UV spectrometry estimation of simvastatin and piperine from pharmaceutical intravenous liquid formulation, the proposed method is very simple, fast, accurate, precise, economical and reproducible.